An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
نویسندگان
چکیده
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
منابع مشابه
Modified acid guanidinium thiocyanate-phenol-chloroform RNA extraction method which greatly reduces DNA contamination.
The most common and consistently successful methods for isolating intact RNA are modifications of the Guanidinium Thiocyanate method of Chirgwin et al. (1). One widely used modification of this method, developed by Chomczynski and Sacchi (2), involves extraction of the guanidinium thiocyanate homogenate with phenol—chloroform at reduced pH. The Acid Guanidinium Thiocyanate Phenol -Chloroform (A...
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1.Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson. 1989. Molecular Biology of the Cell, 2nd ed. Garland Publishing, New York. 2.Atlas, R.M. and L.C. Parks. 1993. Handbook of Microbiological Media, p. 766-767. CRC Press, Boca Raton. 3.Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Bi...
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ورودعنوان ژورنال:
- Analytical biochemistry
دوره 162 1 شماره
صفحات -
تاریخ انتشار 1987